山东地区鸡传染性支气管炎病毒抗原相关性和血清分型

doi:10.11843/j.issn.0366-6964.2015.04.015

Abstract

Abstract:

To clarify the epidemiology of infectious bronchitis virus (IBV) in Shandong province，the antigenic difference among field isolates and vaccine strains was studied.The monovalent antisera against 3 commercial IBV vaccine strains (H120，Ma5 and 4/91) and 18 field isolates were prepared in SPF chickens.The cross viral neutralization (VN) test was performed in SPF chicken embryos to determine the endpoint titers for homologous and heterologous neutralization.The serotype of IBV strains was determined based on antigenic relatedness values (ARV) calculated by the formula R=r1×r2.As a result，vaccine strain 4/91 was belong to serotype I.Vaccine strain H120，Ma5 and two isolates （SDWF0608 and SDLY0701）were grouped in the same serotype.SDZB0808 and the other 15 isolates were grouped in the same serotype.CK/CH/SD09/006 and CK/CH/SD10/001 were in 2 other different serotypes，together with other 3 or 6 IBV isolates，respectively.Moreover，some IBV strains showed one-way neutralizing reaction with others and some IBV strains in the same serotype had different ARVs.The resultes indicate that the antigenicity of IBV strains is very complicated and there are evident antigenic difference between IBV isolates and 3 vaccine strains.

CLC Number:

S852.659.6

HU Bei-xia，XU Chuan-tian，YANG Shao-hua，HUANG Yan-yan，ZHANG Lin，HUANG Qing-hua，HUO Ya-fei，ZHANG Wei，ZHANG Xiu-mei. Antigenic Relatedness and Serotype of Infectious Bronchitis Virus Isolated in Shandong Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 615-623.

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Antigenic Relatedness and Serotype of Infectious Bronchitis Virus Isolated in Shandong Province

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